QuanTI-FRET calibration for quantitative FRET imaging in living samples

Julien Leblanc,Alexis Coullomb,Cécile M. Bidan,Fabian Wehnekamp,Chen Qian,Don C. Lamb,Aurélie Dupont

Advances in Microscopic Imaging III（2021）

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摘要

Quantitative FRET imaging in living cells is made possible on a simple fluorescence microscope with this new method. The single-step calibration is robust and gives absolute FRET values, independent of the instrument.

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