Multicomponent Hydrogel Matrices of Fmoc‐FF and Cationic Peptides for Application in Tissue Engineering

In the last years, peptide-based hydrogels are being increasingly used as suitable matrices for biomedical and pharmaceutical applications, including drug delivery and tissue engineering. Recently, the synthesis and the gelation properties of a small library of cationic peptides, containing a Lys residue at the C-terminus and derivatized with an Fmoc group or with the fluorenyl methoxycarbonyl-diphenylalanine (FmocFF) at the N-terminus are derived. Here, it is demonstrated that the combination of these peptides with the well-known hydrogelator FmocFF, in different weight/weight ratios, allows the achievement of seven novel self-sorted hydrogels, which share similar peptide organization of their supramolecular matrix. Rheological and relaxometric characterization highlight a different mechanical rigidity and water mobility in the gels as demonstrated by the storage modulus values (200 Pa < G' < 35 000 Pa) and by relaxometry, respectively. In vitro studies demonstrate that most of the tested mixed hydrogels do not disturb significantly the cell viability (>95%) over 72 h of treatment. Moreover, in virtue to its capability to strongly favor adhesion, spreading and duplication of 3T3-L1 cells, one of the tested hydrogel may be eligible as synthetic extracellular matrix.