TARGETing Transcriptional Regulation in the Medicinal Plant Catharanthus roseus.

Joana G Guedes, Catarina Leitão, Catarina Meireles,Patrícia Duarte,Mariana Sottomayor

Methods in molecular biology (Clifton, N.J.)（2022）

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摘要

Transcriptional regulation is a central piece of the highly valuable monoterpenoid indole alkaloid pathway of C. roseus , and the ultimate tool for its understanding and manipulation. Here, we describe the adaptation of the TARGET methodology to identify specific and genome-wide leaf targets of C. roseus candidate transcription factors (TFs).

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关键词

Catharanthus roseus,FACS,Monoterpenoid indole alkaloids (MIAs),Protoplasts,TARGET,Transcription factors (TFs)

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