Hierarchical 2D to 3D micro/nano-histology of human dental caries lesions using light, X-ray and electron microscopy

•Nano/macro-histological characterisation of hierarchical structure of human carious enamel performed with various techniques.•Correlative imaging analysis using light microscopy, SEM, FIB and X-ray tomography conducted at different scales.•Qualitative and quantitative 2D and 3D characterisation of the demineralisation of enamel carried out in key zones of carious enamel.•Significant difference in the demineralisation of enamel in the carious lesion and in the surface zone revealed.