Production of Recombinant Human Hybrid Ferritin with Heavy Chain and Light Chain in Escherichia coli and its Characterization.

Xiaotong Song,Yongxiang Zheng,Yongdong Liu,Huan Meng,Rong Yu,Chun Zhang

Current pharmaceutical biotechnology（2023）

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摘要

FTH and FTL subunits were successfully co-assembled into a hybrid ferritin nanoparticle (rhFTH/L). The structure of rhFTH/L was demonstrated highly ordered and fairly compact. Besides, the hybrid rhFTH/L nanoparticle was shown more sensitive to thermal stress and reduced stability when compared with that of both individual rhFTH and rhFTL.

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关键词

E. coli co-expression,Recombinant human hybrid ferritin,heavy chain light chain,human ferritin,mass spectrometry,size exclusion chromatography

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