Detection of dengue virus serotypes by single-tube multiplex RT-PCR and multiplex real-time PCR assay

Kundan Tandel,Mahadevan Kumar,G.S. Bhalla, S.P.S. Shergill,Vijaya Swarnim,Kavita Sahai,R.M. Gupta

Medical Journal Armed Forces India（2022）

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摘要

Prevalence of dengue co-infections is increasing. Moreover, it is important to screen for dengue virus in those samples also which do not show NS1Ag on rapid tests and have either one or both the antibodies. Real-time multiplex RT-PCR is found to be more sensitive in detecting coinfection than conventional multiplex RT-PCR.

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关键词

Dengue,Reverse transcriptase polymerase chain reaction,Real time polymerase chain reaction

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