Non-point source fecal contamination from aging wastewater infrastructure is a primary driver of antibiotic resistance in surface waters

•Widespread correlation of antibiotic resistance genes and human fecal pollution.•Novel synthesis of qPCR and geospatial data to identify contamination sources.•Sewers and aging septic systems are primary drivers of contamination.•Highlights role of non-point sources of antimicrobial resistance to surface waters.