Onion culture: experimental techniques for carrying out high precision experiments

The cultivation of onion Allium cepa L. is of extreme economic importance globally, and, because of this, research must be constantly updated in order to increase productivity. This study was designed to estimate the optimal plot size, sample size and number of repetitions for experiments evaluating variables in the onion crop. Uniformity tests were carried in the years 2016 and 2017 in the experimental area of the Crop Science Department at Universidade Federal de Santa Maria, Santa Maria (RS), Brazil. Each plant was considered a basic experimental unit. Bulb mass, bulb height, and bulb diameter were measured for each basic experimental unit. The optimal plot size, sample size, and number of repetitions were estimated by the method of maximum curvature of the variation coefficient. For onion culture, the optimal plot size to evaluate the bulb mass, bulb height and bulb diameter is eight, six and six, respectively. The optimal sample size for evaluating the bulb mass of the onion crop is six plants, while for the height and diameter variables the optimal sample size is four plants in the direction of the row considering a semi-amplitude of the confidence interval (D%) equal to 20% of the average. Bulb mass variables require 10 repetitions to assess up to 20 treatments in a randomized block design for the least significant difference of the Tukey?? test, expressed as a percentage of the average of 35%. Bulb height and bulb diameter require just six repetitions for the same assessment.