Analysis of polymorphism in 11 STR markers in the European red deer (Cervus elaphus) population in Poland

The aim of this study was to evaluate the levels of variability in selected 11 polymorphic STR markers specific to the red deer (Cerrus elaphus) and their potential use as a tool for identification tests. Biological material was collected from 157 animals during periodic hunting carried out in Poland. Genotyping was conducting using the PCR multiplex method within the range of 11 markers, T115, T501, T156, T193, T108, T107, T172, T507, CSSM19, HAUTI4, and CSSM66, with capillary electrophoresis for product detection. The obtained values of heterozygosity (H-o >0.8), probability of exclusion (PE >0.6), power of discrimination (PD >0.96) and random match probability (RMP <0.06) facilitated assessment of the utility of the marked loci for purposes of identification. Based on die DNA polymorphism in biological material derived from deer, the presented set of 11 markers provides assessment of the degree of inbreeding in the studied animal population, thus combating unfair trade in game and poaching, as well as providing expertise on behalf of the administration of justice.