High Performance Liquid Chromatographic (HPLC) Method for the Estimation of Pitavastatin in Pharmaceuticals

An HPLC method was developed for the monitoring of pitavastatin in pharmaceuticals using C-18 column with detection wavelength at 278 nm. Phosphate buffer, acetonitrile and methanol with the ratio of 30:40:60 was selected as best mobile phase with pH 7.4 and pitavastatin was detected at 278 nm with flow rate of 1 mL/min. The retention time was 3.728 minute while the linearity was determined in the range of 300 to 800 ng/mL with 0.9997 coefficient of correlation. Percentage recoveries in terms of accuracy were in the range of 91.83 to 97.66%. Assay result of pitavastatin in self nanoemulsifying drug delivery system (SNEDDS) was 97.35 %. Method was successfully applied for determination of pitavastatin in developed SNEDDS due to its highest validity.