Polyglutamic Acid-Grafted Halloysite-Modified Collagen-Sodium Alginate Oxide Composite Membrane for Wound Dressing Application

Introducing nanomaterials is an effective strategy for enhancing the mechanical properties of collagen (COL) composite membranes. In this work, we obtained a carboxylated halloysite nanotubes (HNTs) nanomaterial by grafting a macromolecular substance polyglutamic acid (gamma-PGA) on the surface of HNTs, which was coblended with collagen matrix to obtain a collagen-based composite membrane with good mechanical properties under the cross-linking effect of sodium alginate oxide (ADA). We investigated that the gamma-PGA-grafted HNTs have the potential to improve and enhance the tensile strength and elongation at the break of the collagen membranes. A study of contact angle of composite membranes revealed the gamma-PGA-grafted HNTs inhibited the negative effects of HNTs on the hydrophilicity of the COL-ADA membrane. The biological properties of carboxylated HNTs composite-modified collagen-sodium alginate oxide composite membrane (COL-c-ADA) were evaluated in vivo and virto. Polyglutamic acid-grafted HNTs composite-modified collagen-sodium alginate oxide composite membrane material is nontoxic and can promote cell proliferation and migration to promote wound healing.