Expression of Yarrowia lipolytica acetyl-CoA carboxylase in Saccharomyces cerevisiae and its effect on in-vivo accumulation of Malonyl-CoA

Humberto Pereira,Flávio Azevedo,Lucília Domingues,Björn Johansson

Computational and Structural Biotechnology Journal（2022）

引用 8|浏览8

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摘要

•Novel S. cerevisiae strain with tetracycline repressible ACC1 promoter.•Functional expression of Y. lipolytica ACC1 in S. cerevisiae.•Higher malonyl-CoA concentration achieved with Y. lipolytica ACC1 gene.•S. cerevisiae Acc1p seems to interact with the heterologous Y. lipolytica Acc1p.

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关键词

Saccharomyces cerevisiae,ACC1,Fatty acids,Malonyl-CoA,Biosensor,Yarrowia lipolytica

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