In Vivo Investigation of Gallium-68 and Bismuth-205/206 Labeled Beta Cyclodextrin for Targeted Alpha Therapy of Prostaglandin E2 Receptor-Expressing Tumors in Mice.
International journal of pharmaceutics(2022)
Abstract
Prostaglandin E2 (PGE2) molecule and its receptors play an important role in the development of malignancies and metastases therefore PGE2 may play a crucial role in the diagnosis and a new therapeutic target in the field of radionuclide therapy of PGE2-positive tumors. PGE2 form complexes with RAMEB (randomly-methylated-beta-cyclodextrin) with high affinity therefore the aim of this present study was to synthesize a PGE2-specific DOTAGA-RAMEB, which can be labeled with diagnostic and therapeutic isotopes also and binds to PGE2-positive tumors. DOTAGA-RAMEB was labeled with 68Ga and Bi-205/206 radionuclides and their radiochemical purity (RCP%), partition coefficient (logP values), and in vitro and in vivo stability were determined. For the assessment of the biological properties and the PGE2 specificity of [Ga-68]Ga-DOTAGA-RAMEB and [Bi-205/206]Bi-DOTAGA-RAMEB in vivo PET imaging and ex vivo biodistribution studies were performed using healthy control and PGE2-positive BxPC-3 tumor-bearing CB17 SCID mice. The RCP% of the newly synthesized [Ga-68]Ga-DOTAGA-RAMEB and [Bi-205/206]Bi-DOTAGA-RAMEB was higher than 98 %. In vivo studies showed that the tumor-to-background ratio of [Ga-68]Ga-DOTAGA-RAMEB was 2.5 +/- 0.2 as a result BxPC-3 tumors were clearly identified on PET images. Beside this the ex vivo biodistribution studies showed that the accumulation rate of [Ga-68]Ga-DOTAGA-RAMEB and [Bi-205/206]Bi-DOTAGA-RAMEB was similar in the PGE2-positive BxPC-3 tumors.
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Key words
Radiotherapy,Cyclodextrin,Targeted alpha therapy,Prostaglandin E2,Pancreas adenocarcinoma
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