Immunoinformatics Evaluation of a Fusion Protein Composed of Leishmania infantum LiHyV and Phlebotomus kandelakii Apyrase as a Vaccine Candidate against Visceral Leishmaniasis

& nbsp;Background: Visceral leishmaniasis (VL) is a lethal parasitic disease, transmitted by sand fly vectors. Immunomodulatory properties of sand fly saliva proteins and their protective effects against & nbsp;Leishmania& nbsp;infection in pre-exposed animals suggest that a combination of an antigenic salivary protein along with a & nbsp;Leishmania& nbsp;antigen can be considered for designing a vaccine against leishmaniasis Methods: Three different fusion forms of & nbsp;L. infantum& nbsp;hypothetical protein (LiHyV) in combination with & nbsp;Phlebotomus kandelakii& nbsp;salivary apyrase (PkanAp) were subjected to & nbsp;in-silico& nbsp;analyses. Major Histocompatibility Complex (MHC) class I and II epitopes in both humans and BALB/c mice were predicted. Antigenicity, immunogenicity, epitope conservancy, toxicity, and population coverage were also evaluated. Results: Highly antigenic promiscuous epitopes consisting of truncated LiHyV (10-285) and full-length PkanAp (21-329) were identified in human and was named Model 1. This model contained 25 MHC-I and 141 MHC-II antigenic peptides which among them, MPANSDIRI and AQSLFDFSGLALDSN were fully conserved. LALDSNATV, RCSSALVSI, ALVSINVPL, SAVESGALF of MHC-I epitopes, and 28 MHC-II binding epitopes showed 60% conservancy among various clades. A population coverage with a rate of > 75% in the Iranian population and > 70% in the whole world was also identified. Conclusion: Based on this & nbsp;in-silico& nbsp;approach, the predicted Model 1 could potentially be used as a vaccine candidate against VL.