Isolation and biosynthesis of daturamycins from Streptomyces sp. KIB-H1544

Two novel diarylcyclopentenones daturamycin A and B (1 and 2), and one new p-terphenyl daturamycin C (3), along with three known congeners (4-6), were isolated from a rhizosphere soil-derived Streptomyces sp. KIB-H1544. The structures of new com-pounds were elucidated via a joint use of spectroscopic analyses and single-crystal X-ray diffractions. Compounds 1 and 2 belong to a rare class of tricyclic 6/5/6 diarylcyclopentenones, and compounds 3-6 possess a C-18 tricyclic aromatic skeleton. The biosyn-thetic gene cluster of daturamycins was identified through gene knockout and biochemical characterization experiments and the biosynthetic pathway of daturamycins was proposed.