Alkaline Comet Assay to Detect DNA Damage.
Methods in molecular biology (Clifton, N.J.)(2023)
摘要
The comet assay is an effective method for identifying DNA breaks and alkali-labile sites induced by genotoxins. Performed as a single-cell electrophoresis, this assay is especially simplistic, and the results are easily reproducible. DNA breakage can be quantitatively assessed by the induced comet tail regions, which can be measured using a variety of comet software. This protocol will finish within approximately two hours with adequate preparation, and digitized images can be taken using a confocal or standard fluorescence microscopes after staining the cell nucleus with a DNA dye.
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关键词
Alkaline lysis,Comet assay,Single-cell electrophoresis
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