Correction: High-Throughput Neurite Outgrowth Assay Using GFP-Labeled iPSC-Derived Neurons.

Current Protocols is issuing corrections for the following Protocol article. Zhang, L., Li, S., & Xia, M. (2022). High-throughput neurite outgrowth assay using GFP-labeled iPSC-derived neurons. Current Protocols, 2, e542. doi: 10.1002/cpz1.542. Article Introduction, 4th paragraph (p.2): The sentence: “While tubulin staining requires multiple washing steps, which is time-consuming and increases well-to-well variation, calcein AM staining also requires washing steps and in addition cannot be used for time-lapse imaging due to cytotoxicity.” has been edited to read: “While tubulin staining requires multiple washing steps, which is time-consuming and increases well-to-well variation, calcein AM can be used for live staining (no wash steps involved), but still cannot be used for time-lapse imaging due to cytotoxicity.” Commentary, Background Information, 2nd paragraph (p. 10): The sentence: “However, calcein AM staining also requires time-consuming washing steps.” has been edited to read: “However, the calcein AM method still requires time-consuming staining steps.” The current version online now includes these corrections and may be considered the authoritative version of record.