Phytochemical investigation and cytotoxicity study of Indigofera zollingeriana crude extract

This study aimed to identify the phytochemical constituents in Indigofera zollingeriana (I.zollingeriana) leaves crude extracts and their cytotoxicity activity against different cell lines. The I.zollingeriana leaves were extracted using the cold maceration method, where the leaves were macerated in the ethanol, acetonitrile, acetone, and chloroform separately at room temperature. The total phenolic content (TPC), total tannin content (TTC) and total flavonoid content (TFC) of the crude extract were determined spectrophotometrically. Ethanol extract gave a higher value of TPC, TTC, and TFC (71.38, 50.06, and 8.43 mg/g, respectively) compared to the other solvents. In GC-MS analysis, ethanol extract showed the presence of 59 compounds, while LC-MS analysis showed the abundant presence of triphenylphosphine oxide (95.00%). The cytotoxicity of the ethanol crude extract against different cell lines namely human lung fibroblast (MRCS), African green monkey kidney (Vero), human epithelium lung adenocarcinoma (A549) and human breast cancer (MCF-7) cell lines were determined. The cytotoxicity results revealed that the IC50 for ethanol extract against cancer cells (A549 and MCF-7) were 49.26 and 75.70 mu g/ml, respectively. The IC50 value for ethanol extract against Vero cells was 58.98 mu g/ml, while for MRCS cells was declared non-toxic with IC50 value was > 100.00 mu g/ml. Copyright (C) 2022 Elsevier Ltd. All rights reserved.