Phosphorothioate oligonucleotides separation in ion-pairing reversed-phase liquid chromatography: effect of temperature

Journal of Chromatography A(2022)

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摘要
•IP RP LC separation of PS ONs was investigated in temperature range 20 – 90 °C.•Separation of PS ONs diastereomers is suppressed at high temperature.•Suppression of isomeric separation leads to narrower peaks.•n/n-x resolution of PS ONs is enhanced at elevated temperature.•Higher temperature does not lead to ONs retention decrease for all IP systems.
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关键词
Phosphorothioate oligonucleotides,Ion-pairing reversed-phase chromatography,Diastereomeric separation,Temperature,Resolution
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