Structural rearrangements of a caspase-like protease TPR-CHAT govern virus-host discrimination during type III-E CRISPR-Caspase immunity

The RNA-targeting type III-E CRISPR-gRAMP effector forms a complex with a caspase-like protease TPR-CHAT, but the mechanistic details of their functional relationship remain unknown. Here, we report on cryo-EM structures of gRAMPcrRNA and gRAMPcrRNA-TPR-CHAT complexes, before and after either self or non-self RNA target binding, elucidating mechanisms underlying RNA-targeting and non-self RNA-induced protease activation. Noteworthy, the associated TPR-CHAT adopts a strikingly distinct conformation on self versus non-self RNA targets, with nucleotides at position −1 and −2 of crRNA serving as a sensor. Only binding of non-self RNA target activates TPR-CHAT protease, leading to the cleavage of Csx30 protein. Furthermore, given that TPR-CHAT structurally resembles eukaryotic separase, our results implicate an ancient mechanism for separase regulation. Our findings should not only facilitate the development of gRAMP-based RNA manipulation tools, but also lead to a mechanistic understanding of the virus-host discrimination process governed by a caspase-like protease during type III-E CRISPR-Caspase immunity. ### Competing Interest Statement The authors have declared no competing interest.