New insights into P2X7 receptor regulation: Ca2+-calmodulin and GDP bind to the soluble P2X7 ballast domain

P2X7 receptors are non-selective cation channels that are activated by extracellular ATP and play important roles in inflammation. They differ from other P2X family members by a large intracellular C-terminus that mediates diverse signaling processes that are little understood. A recent cryo-EM study revealed that the C-terminus of the P2X7 receptor forms a unique cytoplasmic ballast domain that possesses a GDP-binding site as well as a dinuclear Zn site. However, the molecular basis for the regulatory function of the ballast domain as well as the interplay between the various ligands remains unclear. Here, we successfully expressed a soluble trimeric P2X7 ballast domain (P2X7BD) and characterized its ligand binding properties using a biophysical approach. We identified calmodulin-binding regions within the ballast domain and found that binding of Ca-calmodulin (Ca-CaM) and GDP to P2X7BD have opposite effects on its stability. Small-angle X-ray scattering (SAXS) experiments indicate that Ca-CaM binding disrupts the trimeric state of P2X7BD. Our results provide a possible framework for the intracellular regulation of the P2X7 receptor.