Effects of Chitosan Oligosaccharide on Production Performance, Egg Quality and Ovarian Function in Laying Hens with Fatty Liver Syndrome

Simple Summary Fatty liver syndrome (FLS) often occurs in caged laying hens and can cause decreases in production performance. Chitosan oligosaccharide (COS), degraded from chitin or chitosan, has been demonstrated to prevent metabolic diseases in rodents. In this work, we found that dietary COS supplementation could improve production performance and egg quality in laying hens with FLS. Further study indicated that improved ovarian morphology and function may be involved in these beneficial effects of COS. Specifically, dietary COS supplementation decreased oxidative stress, inflammation and apoptosis in the ovaries of laying hens with FLS. This study provides evidence for the application of COS to improve production performance and egg quality in laying hens with FLS. This study aimed to investigate the role of chitosan oligosaccharide (COS) as an additive in the feed of laying hens with fatty liver syndrome (FLS). Effects on production performance, egg quality as well as ovarian function were determined. A total of 360 Lohmann Pink-shell laying hens (28 weeks old) were randomly assigned to 5 groups (6 replicates x 12 birds). Hens were fed with a basal diet and a high-energy low-protein (HELP) diet supplemented with 0, 200, 400 and 800 mg/kg COS. COS reversed the lowered laying rates, increased feed-to-egg ratios and decreased albumen heights and Haugh units induced by the HELP diet. Additionally, COS improved the ovarian morphologies damaged by the HELP diet. Furthermore, COS enhanced antioxidant enzyme activities, reduced malonaldehyde levels and downregulated the mRNA expressions of nuclear factor kappa B, pro-inflammation cytokine genes and pro-apoptosis-related genes, while it upregulated the mRNA expression of anti-apoptosis-related genes in the ovaries of HELP-diet-fed hens. These findings suggested that dietary COS supplementation could improve production performance and egg quality in laying hens with FLS, and these beneficial effects were linked to improved ovarian morphology, which was attributed to decreased oxidative stress, inflammation and apoptosis in the ovaries.