Autofluorescence Label-Free Imaging of the Liver Reticular Structure.

Methods in molecular biology (Clifton, N.J.)(2023)

引用 1|浏览5
暂无评分
摘要
Autofluorescence rising from biological substrates under proper excitation light depends on the presence of specific endogenous fluorophores and can provide information on the morpho-functional properties in which they are strictly involved. Besides the numerous endogenous fluorophores involved in metabolic functions, fibrous proteins may act as direct, label-free biomarkers of the tissue structural organization. The optical properties of collagen, in particular, are currently applied as an alternative to established histochemical procedures to investigate the connective tissue as well as its changes in diseased conditions. This is particularly true in hepatology where the histochemical procedures to label the reticular structure are not routinely applied, as they are complex and time-consuming. The morphology of the liver reticular structure and its changes are up to now poorly considered despite the increasing awareness of the regulatory role played by the remodeling of the reticular structure in pathological conditions. In this context, the autofluorescence label-free imaging has proven to be a suitable approach.
更多
查看译文
关键词
Collagen,Endogenous fluorophore,Fatty liver,Sinusoids
AI 理解论文
溯源树
样例
生成溯源树,研究论文发展脉络
Chat Paper
正在生成论文摘要