A new platform host for strong expression under GAL promoters without inducer in Saccharomyces cerevisiae

•An alternative host strain (allgal) was developed for the production of recombinant proteins in S. cerevisiae.•For efficient expression of GAL promoters without expensive inducer, all genes related to the galactose metabolism was removed.•The growth of the allgal mutant was enhanced by 15–38% compared to the gal80 mutant, and the secretion of recombinant proteins also increased by 16–22% in fed-batch fermentation.•The expression of recombinant proteins using GAL10 promoter in the allgal mutant is suitable for the economical production of recombinant proteins in S. cerevisiae.