Enhanced anaerobic digestion of corn stover using magnetized cellulase combined with Ni-graphite coating

The effects of combining magnetized cellulase (MC) with Ni-graphite (NG) coated materials on the anaerobic digestion of corn stover were investigated. In pretreatment of the corn stover, cellulase showed higher glycosylation efficiency in a 30 mT magnetic field environment than in other magnetic fields, and the reducing sugar content reached 0.182 mg/L. Treatment with the combination of magnetized cellulase and Ni-graphite coated material resulted in a 74.35% increase in cumulative CH4 yield. Analysis of the composition of the microbial community showed that the addition of Ni-graphite coating significantly influenced the composition of the microbial community. The electrically active microorganisms Bacteroidota (46.98%) and Methanomicrobiales (6.08%) became the dominant bacteria and archaea when the corn stover was treated with the NG+MC combination. Carbon balance calculations showed that the combination treatment resulted in carbon flow distributions that differed from other treatments, and the energy conversion efficiency (ECE) reached 57.1%.