Rapid lignin quantification for fungal wood pretreatment by ATR-FTIR spectroscopy

Nikolett Wittner,János Slezsák,Waut Broos,Jordi Geerts,Szilveszter Gergely,Siegfried E Vlaeminck,Iris Cornet

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy（2023）

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摘要

•ATR-FTIR method development for easy monitoring of fungal lignin degradation.•Calibration data set from > 40 solid-state fermentation set-ups.•Washing is necessary to prevent interferences of proteins and fungal biomass.•A PLSR model with very good performance indicators was achieved.•Greatly reduced analysis time (<10 min) compared to wet chemistry methods (>3h)

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关键词

Biobased economy,Lignin analysis,Manganese peroxidase,Phanerochaete chrysosporium,Solid-state fermentation,White-rot fungi

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