The alarmin interleukin-1 alpha triggers secondary degeneration through reactive astrocytes and endothelium after spinal cord injury
Nature communications(2022)
摘要
Spinal cord injury (SCI) triggers neuroinflammation, and subsequently secondary degeneration and oligodendrocyte (OL) death. We report that the alarmin interleukin (IL)-1 alpha is produced by damaged microglia after SCI. Intra-cisterna magna injection of IL-1 alpha in mice rapidly induces neutrophil infiltration and OL death throughout the spinal cord, mimicking the injury cascade seen in SCI sites. These effects are abolished through co-treatment with the IL-1R1 antagonist anakinra, as well as in IL-1R1-knockout mice which demonstrate enhanced locomotor recovery after SCI. Conditional restoration of IL-1R1 expression in astrocytes or endothelial cells (ECs), but not in OLs or microglia, restores IL-1 alpha-induced effects, while astrocyte- or EC-specific Il1r1 deletion reduces OL loss. Conditioned medium derived from IL-1 alpha-stimulated astrocytes results in toxicity for OLs; further, IL-1 alpha-stimulated astrocytes generate reactive oxygen species (ROS), and blocking ROS production in IL-1 alpha-treated or SCI mice prevented OL loss. Thus, after SCI, microglia release IL-1 alpha, inducing astrocyte- and EC-mediated OL degeneration.
更多查看译文
AI 理解论文
溯源树
样例
生成溯源树,研究论文发展脉络
Chat Paper
正在生成论文摘要