Two-field transverse relaxation-optimized spectroscopy for the study of large biomolecules – An in silico investigation

•Optimal relaxation properties can be found at vastly different magnetic fields for two nuclear spins in the same spin system.•Two-field transverse relaxation optimized experiments can benefit from excellent relaxation properties for both nuclei within a single experiment.•Numerical simulations of 13C-19F pairs in aromatic side-chains of large proteins show an increase of sensitivity >x10 and better resolution with two-field methods.