Glucoregulatory Reprogramming in Male Mice Offspring Induced by Maternal Transfer of Indoor Flame Retardant Endocrine Disruptors
biorxiv(2022)
摘要
Polybrominated diphenyl ethers (PBDEs) are commercially used as indoor flame retardants that penetrate biota and bioaccumulate in human tissues, including breast milk. PBDEs have been associated with endocrine disruption, diabetes and metabolic syndrome (MetS) in humans and animals. However, their sex-specific diabetogenic effects are not completely understood. Our past works show diabetogenic effects of the commercial penta-mixture of PBDEs, DE-71, in perinatally exposed C57Bl/6 female mice. As a comparison, in the current study, the effects of DE-71 on glucose homeostasis in male offspring were examined. C57BL/6 dams were exposed to DE-71 at 0.1 mg/kg/d (L-DE-71), 0.4 mg/kg/d (H-DE-71) or received corn oil vehicle (VEH/CON) for a total of 10 wks, including gestation and lactation. Male offspring were examined in adulthood and DE-71 exposure produced hypoglycemia upon extended fasting. In vivo glucose challenge testing showed marked intolerance (H-DE-71) and incomplete clearance (L- and H-DE-71). Moreover, L-DE-71-exposed mice showed altered glucose responses to insulin, especially incomplete glucose clearance and/or utilization. In addition, L-DE-71 produced elevated levels of plasma glucagon and the incretin GLP-1 but no changes were detected on insulin. These alterations, which represent relevant criteria used clinically to diagnose diabetes, were accompanied with reduced hepatic glutamate dehydrogenase enzymatic activity, elevated adrenal epinephrine and decreased thermogenic brown adipose tissue mass, which may indicate several organ system targets of PBDEs. Liver levels of several endocannabinoid species were not altered by perinatal exposure to DE-71 in males. Our findings demonstrate that chronic low exposure to PBDEs in mothers can reprogram glucose homeostasis and glucoregulatory hormones in male offspring. Previous findings using female offspring showed altered glucose homeostasis that aligned with a contrasting diabetogenic phenotype. We summarize the results of the current work generated in males in light of previous findings on females. Taken together, these findings, combined with our prior results, offer a comprehensive account of sex-dependent effects of maternally transferred environmentally relevant PBDEs on glucose homeostasis and glucoregulatory endocrine dysregulation.
### Competing Interest Statement
The authors have declared no competing interest.
* 2-AG
: 2-arachidonoyl-sn-glycerol
2-DG
: 2-docosahexaenoyl-sn-glycerol
AEA
: arachidonoylethanolamide
AUC
: area under curve
BAT
: brown adipose tissue
BDE(s)
: decabromodiphenyl ethers
DE-71
: pentabrominated diphenyl ether mixture
DHEA
: docosahexaenoyl ethanolamide
EC
: endocannabinoid
EDC(s)
: endocrine disrupting chemicals
EDTA
: ethylenediamine tetraacetic acid
EIA/ELISA
: enzyme-linked immunosorbent assay
EPA
: Environmental Protection Agency
FBG
: fasting blood glucose
GDH
: glutamate dehydrogenase
GLP-1
: glucagon-like peptide 1
GTT
: glucose tolerance test
ITT
: insulin tolerance test
LOEL
: lowest-observed-adverse-effect level
MDC(s)
: metabolism-disrupting chemicals
MetS
: metabolic syndrome
OEA
: n-oleoylethanolamide
PBDE(s)
: polybrominated diphenyl ethers
PND
: postnatal day
POPs
: persistent organic pollutants
T2D
: type 2 diabetes
UPLC/MS/MS
: ultra-high performance liquid chromatography with tandem mass spectrometry
VEH/CON
: vehicle control group
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关键词
male mice offspring induced,glucoregulatory reprogramming,endocrine,maternal transfer
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