Radiocomplexation, Chromatographic Separation and Bioevaluation of [ 99m Tc]Dithiocarbamate of Procainamide as Selective Labeled Compound for Myocardial Perfusion Imaging

Cardiac imaging is one of the most important tools for diagnosing many diseases related to the heart. This work was devoted to developing this method using experiments on mice. The formation of a complex of [ 99m Tc]dithiocarbamate of procainamide under optimum conditions of reaction temperature (37°C), reaction time (30 min), pH of the reaction mixture (8), amount of substrate (100 μg), amount of reducing agent tin(II) (content, 50 μg), and stability in rat serum (8 h) was achieved using radioactive Tc-99m (300 – 500 MBq) to give high labeling yield (98.5%) and radiochemical purity. Procainamide works through sodium channel blocker. Normal mice were used in biodistribution investigations. High absorption uptake of the [ 99m Tc]dithiocarbamate of procainamide complex was found to be 33.47 ± 0.83% injected dose/g organ (ID/g) as observed in mice for up to 5 min, which demonstrated its usefulness as a radiotracer for heart imaging.