Tristaenone A: A New Anti-Inflammatory Compound Isolated from the Australian Indigenous Plant Tristaniopsis laurina

Inspired by ethnopharmacological knowledge, we conducted a bioassay-guided fractionation of the leaves of Tristaniopsis laurina which led to the discovery of a new anti-inflammatory compound, tristaenone A (1). The structure was elucidated by detailed spectroscopic data analysis, and the absolute configuration was established using X-ray crystallography analysis. Tristaenone A (1) suppressed LPS and IFN-gamma-induced NO, TNF-alpha and IL-6 production in RAW 264.7 cells with IC50 values of 37.58 +/- 2.45 mu M, 80.6 +/- 5.82 mu M and 125.65 +/- 0.34 mu M, respectively. It also inhibited NF-kappa B nuclear translocation by 52.93 +/- 14.14% at a concentration of 31.85 mu M.