Single-cell RNA sequencing analysis revealed cellular and molecular immune profiles in lung squamous cell carcinoma

•TIGIT, a novel immunosuppressive receptor, was identified in Tregs and exhausted CD8+ T cells.•TAN (tumor-associated neutrophil) was identified and able to contribute to an immunosuppressive TME in LUSC.•Myofibroblast was associated with survial. Trajectory analysis revealed that Myofibroblast was differentiated from PDGFRA/B+ fibroblast.•SPP1+ macrophage was correlated with survival and promoted tumor progression in TME.•SPP1+ macrophage based regulatory networks were constructed. The pattern of SPP1-CD44 might be the potential target for LUSC.