Identification and functional comparison of primary astrocytes and microglia

Jing-Ming Shi, Hang Liu, Long Cai,Yi-Bo Guo, Hui Ma,Yan-Song Li,Sha-Di Li,Ze-Yu Zhang, Yan-Dong Ma, Cheng-Hui Guo

Journal of Neuroscience Methods（2023）

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摘要

•Appropriate primary and secondary antibody pairs are essential for identifying glia cells.•The mild patting techniques are efficient for purifying microglia.•Microglia and astrocytes react differently to LPS-stimulation.

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关键词

GFAP antibody,Iba1 antibodies,Flapping methods,Lipopolysaccharide,Cytokines

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