Atypical Lesions in Canine Leishmaniosis: Description of New Cases

Simple Summary Canine leishmaniosis is a zoonotic disease caused mainly by the protozoan Leishmania infantum that is endemic in the Mediterranean basin. The clinical signs and pathological characteristics have been extensively described; however, atypical lesions leading to clinical and pathological misdiagnosis have been sporadically reported. This study presents three cases of dogs experimentally infected with Leishmania infantum that showed gross lesions considered atypical of canine leishmaniosis and not previously reported. Macroscopic lesions were observed in the liver, spleen, and peritoneal cavity that corresponded to tan to whitish nodular lesions in all organs and multifocal irregular and linear whitish lesions in one of the livers that mimicked tumors. Microscopically, a marked granulomatous inflammation with a relative scarcity of parasites was observed in all the organs. In conclusion, microscopic and parasitological studies should be performed on atypical lesions observed in Leishmania-infected and non-infected dogs, especially in endemic regions. In addition, due to its zoonotic importance, leishmaniosis should be considered when atypical nodular lesions are observed in dogs from non-endemic regions with a history of travel to endemic regions. Pathological characteristics are well described in canine leishmaniosis (CanL). However, atypical lesions that can be confused with other pathologies or trigger unusual clinical signs are sporadically reported. Atypical lesions were observed during routine postmortem examination in three Leishmania-infected dogs and samples were taken for histopathological and immunohistochemical studies. Clinical signs, biochemical parameters, level of antibodies, and parasite detection by PCR were also evaluated. Atypical lesions were found in the peritoneal cavity, liver, and spleen. Splenomegalia and hepatomegalia were observed in all dogs. In addition, multifocal dark to white nodules of variable size were observed in the peritoneal cavity, liver, and spleen of one dog and in the spleen of the other two dogs. One dog presented diffuse irregular whitish lines with a threadlike appearance and another an intense fibrotic depression in the intermediate lobe. Microscopically, an intense granulomatous inflammation with abundant macrophages, a variable number of lymphocytes, and a low to moderate number of parasites was observed. This study represents the first description of granulomatous peritonitis associated with Leishmania in dogs. It also shows atypical macroscopic expression of hepatitis in CanL. In the absence of an adequate clinical history and laboratory analyses, certain lesions observed in CanL could admit alternative diagnoses.