Characterization by Polyphasic Approach of Some Indigenous Halophilic Archaea of Djelfa’s Rock Salt “hadjr El Meelh”, Algeria

Background and Objectives: Hadjr El Melh of Djelfa is an example of hypersaline ecosystems, which can harbor a wide variety of microorganisms under hostile physicochemical conditions. Given the importance of the study of halophilic mi- croorganisms present there in terms of fundamental and applied microbiology, the purpose of this study was to characterize some halophilic archaea isolated from the brines of this environment. Materials and Methods: Eight water samples were chosen randomly and collected for physicochemical and microbiolog- ical analyses. Isolation of halophilic archaea was carried out by membrane filter technique. Ten strains were identified by polyphasic approach and tested for enzymes production. Results: Water samples of Djelfa’s rock salt were slightly acidic to neutral in pH (6.55-7.36) with salinity ranging from 258.68 g/l to 493.91 g/l. Phenotypic, biochemical, taxonomic and phylogenetic characteristics indicated that all strains were classified within the family of Halobacteiaceae. Based on the comparison of DNA sequences encoded 16S rRNA, it was determined that seven strains were affiliated to the genus Haloarcula, two strains were related to the genus Halobacterium and one strain within the genus Haloferax. Production of different enzymes such as protease, amylase, esterase, lipase, leci- thinase, gelatinase and cellulase on solid medium indicated that one strain (S2-2) produced amylase, esterase, lecithinase and protease. However, no strains showed cellulolytic or lipolytic activity. Gelatinase was found in all tested strains. Conclusion: This report constitutes the first preliminary study of culturable halophilic archaea recovered from the brines of Djelfa’s rock salt with a promising enzymatic potential in various fields of biotechnology.