How different farming practices influence the activity of insectivorous Neotropical bats

•We examined the activity and sonotypes of aerial insectivorous bats present in a human-modified landscape in Southeast Brazil.•Bats exhibited reduced activity and richness in Eucalyptus plantations.•Forest canopy density had a negative effect on overall activity and sonotypes richness of aerial insectivorous bats.•This study reinforce the importance of maintaining preserved areas of native vegetation in agropastoral landscapes.