Global change may make hostile – Higher ambient temperature and nitrogen availability increase ant aggression

Behaviour is a response of organisms to internal and external stimuli and comprises various activities such as searching for food. Aggression is important in such activities, for example, improving the chances of winning competition for food, but animals differ in their level of aggression. This behavioural plasticity allows individuals to respond to environmental changes and is important for the survival of animals. It may be an important asset in facing global changes, which affect all organisms, for example, via rising temperature and eutrophication. The latter have steadily increased since 1900, especially in high elevations. Their effects may first become visible in stationary organisms such as ants because their nests are strictly associated with the conditions on site. Here, we analysed eight populations of the high-elevation ant Tetramorium alpestre along several elevations spanning the European Alps. We conducted a correlative approach and analysed several genetic and environmental proxies, namely within- and across-colony genetic relatedness, cuticular hydrocarbons, body size, across-colony geographic distance, air temperature, and worker nitrogen values additionally to within-population aggressive behaviour. We hypothesised that a) these proxies and aggressive behaviour differ among populations and that b) one or more of these proxies influence aggression. We found that a) some environmental proxies and aggression differed among populations but not the genetic proxies and that b) air temperature and worker nitrogen-isotope values correlated positively with worker aggression. The results indicate an environmental but not social-structural influence on this ant's aggressive behaviour, even though social structure varied among populations (single- and multiple-queened colonies). We infer that global change affects aggression in our study system and propose five mutually non-exclusive scenarios to explain the behavioural change mechanistically. Using the space-for-time principle, we speculate that aggression may increase due to future increases in temperature and nitrogen availability in this ant and other species living in high elevations.