RNA interference of vATPase subunits A and E affects survival of larvae and adults in Plagiodera versicolora (Coleoptera: Chrysomelidae)

Vacuolar-type H+-ATPases (vATPases) are ATP-driven proton pumps and play essential roles in many physiological functions. Plagiodera versicolora (Coleoptera: Chrysomelidae) is a leaf-eating forest pest found in salicaceous trees worldwide. RNA interference (RNAi) is a powerful tool for functional identify and pest control. In this study, we used RNAi as an approach to knock down subunits A and E of the vATPase gene. The phylogenetic analysis showed that vATPase-A and vATPase-E from the same order were clustered together to form Coleoptera subclades, respectively. The expression levels of vATPase-A and vATPase-E were higher in gut, Malpighian tubules and 1st instar larvae. Ingest the dsvATPase-A and dsvATPase-E significantly inhibited the development of 1st to 3th instar larvae, incapacitated of mating and oviposition in adults. In addition, knockdown of vATPase subunit genes caused higher mortality in larvae and adults. The results demonstrate that RNAi efficiencies both vATPase-A and vATPase-E genes at various larvae stages and adults. Moreover, this research suggested that silencing of two vATPase subunits A and E offers a potential strategy to control P. versicolora.