Quantification of Lactoferrin in Human Milk Using Monolithic Cation Exchange HPLC

•Monolithic cation exchange HPLC proved to be a fast and efficient method for the quantification of lactoferrin in human milk.•Lactoferrin concentrations did not correlate with the milk collection method, i.e., by manual expression or a breast pump.•The concentration of lactoferrin in human milk was negatively correlated with the stage of lactation.