Spatially Characterizing the Immune Contexture in Mouse Tissue Using Multiplex Immunohistochemistry.
Methods in molecular biology (Clifton, N.J.)（2023）
摘要
Multiplex immunohistochemistry (mIHC) facilitates the simultaneous detection of various immune cell markers on a single tissue section. Here, we describe a protocol for an mIHC staining workflow using specific antibodies against CD4, CD8α, FOXP3, and B220 to identify distinct lymphocyte populations including T and B cells. This staining strategy can be adapted to include other cell markers to evaluate the immune contexture in murine tissues.
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关键词
Cell surface markers,Immune cells,Multiplex immunohistochemistry,Spatial phenotyping,Tyramide signal amplification (TSA)
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