Effects of diflubenzuron on shrimp (Neocaridina palmata) in freshwater systems dominated by submerged plant (Ceratophyllum demersum).

Diflubenzuron (DFB) is a benzoylbenzourea insect growth regulator widely used in agriculture, horticulture, and vector control. Therefore, it can easily pollute water bodies and cause harm to aquatic life and ecosystems. To evaluate the impact of DFB on atyid shrimp Neocaridina palmate, the insecticide was applied, at 0, 0.74, 2.222, 6.667, 20, and 60 μg L, to indoor systems dominated by submerged plant Ceratophyllum demersum. The highest no observed effect concentration and the lowest observed effect concentration was determined to be 0.167 and 0.536 μg L, respectively, as it was counted with either activity or immune-reactive content of chitobiase. Subcellular indices were more sensitive, with a lowest observed effect concentration below 0.107 μg L. Principal response curves (PRC) and principal component analysis (PCA) showed that DFB reduced the biomass of C. demersum and the content of chlorophyll-a and phycocyanin in the media. The biomass of periphyton were promoted at the high concentrations. According to the PRC and PCA, DFB reduced the bacterial population related to photoautotrophy, sulphur reduction, and sulphur oxidation and it promoted those related to photoheterotrophy, nitrate reduction, nitrate denitrification, and nitrogen fixation. Besides, DFB reduced fungi related to denitrification. PRC and PCA showed that DFB had a negative impact on pH and dissolved oxygen levels and a positive impact on NH-N, NO-N, PO-P, and conductivity, suggesting the deterioration in quality of water. This study provided useful information for understanding the ecotoxicological effects of DFB at population and community levels.