Aeromonas species isolated from aquatic organisms, insects, chicken, and humans in India show similar antimicrobial resistance profiles.

species are Gram-negative bacteria that infect various living organisms and are ubiquitously found in different aquatic environments. In this study, we used whole genome sequencing (WGS) to identify and compare the antimicrobial resistance (AMR) genes, integrons, transposases and plasmids found in , and isolated from Indian major carp (, Indian carp (, catfish () and Nile tilapia () sampled in India. To gain a wider comparison, we included 11 whole genome sequences of spp. from different host species in India deposited in the National Center for Biotechnology Information (NCBI). Our findings show that all 15 sequences examined had multiple AMR genes of which the Ambler classes B, C and D β-lactamase genes were the most dominant. The high similarity of AMR genes in the sequences obtained from different host species point to interspecies transmission of AMR genes. Our findings also show that all sequences examined encoded several multidrug efflux-pump proteins. As for genes linked to mobile genetic elements (MBE), only the class I integrase was detected from two fish isolates, while all transposases detected belonged to the insertion sequence (IS) family. Only seven of the 15 sequences examined had plasmids and none of the plasmids encoded AMR genes. In summary, our findings show that spp. isolated from different host species in India carry multiple AMR genes. Thus, we advocate that the control of AMR caused by spp. in India should be based on a One Health approach.