Genome-wide characterization of the inositol transporters gene family in Populus and functional characterization of PtINT1b in response to salt stress

Inositol transporters (INTs) can mediate the transmembrane transport of inositol, and play crucial roles in plant growth, development and stress resistance. However, the INT gene family in Populus has not been reported. Herein, nine INT genes were identified in the Populus trichocarpa genome and divided into three clades. Tandem duplication and whole-genome duplication events could induce the expansion of PtINT gene family. It was worth noting that PtINT1c* and 1d* formed by twice tandem gene duplication events of PtINT1b, but both had un-dergone partial structural loss during evolution. PtINT2_p1* and PtINT2_p2* might be originated from one INT2 gene by stop codon-and start codon-gain variants. Different members of PtINTs were localized to the plasma membrane or vacuolar membrane. PtINTs had diversified tissue expression profiles, and many members were significantly induced or suppressed after salt and drought treatments. PtINT1b was induced by drought and salinity stresses, and encoded a vacuolar inositol transporter. Overexpression of PtINT1b rendered the transgenic Arabidopsis plants more resistant to salt stress. In conclusion, this study provides valuable clues for future research on the function of PtINTs, and PtINT1b was identified as a candidate gene for genetic engineering to enhance salinity tolerance in plants.