GoldenFish: a rapid and efficient system to customize constructs for zebrafish transgenesis.

Zhanmei Jiang, Lirong Huang,Jieqiong Zhao,Yanfeng Li,Jianlong Ma,Rui Ni,Qifen Yang,Lingfei Luo,Yun Yang,Jingying Chen

Journal of molecular cell biology（2023）

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摘要

Transgenesis, which inserts exogenous DNA into animal genomes, is a widely used technique. Traditionally, the con- structs for transgenesis are generated by step-by-step subcloning of DNA frag- ments, which requires multiple steps depending on the construct complexity.

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关键词

Golden Gate cloning,module,transgenesis,zebrafish

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