In-vitro anticoccidial evaluation of citrus sinensis essential oil against eimeria oocysts

Poultry coccidiosis has been conventionally controlled by various anticoccidial chemicals. But, due to multiple issues associated with chemicals, need for safe alternates is crucial and essential oils are among the best candidates. Hence, the current in-vitro research was conducted to examine the anticoccidial potential of Citrus sinensis essential oil against mixed species of Eimeria oocysts. In this experiment, the anticoccidial activity was estimated by sporulation inhibition method. Eight groups were made based on different concentrations of C. sinensis essential oil and other control chemicals. The results revealed a dose-dependent response with maximum sporulation inhibition activity at the highest concentration of the C. sinensis essential oil. Thus, this essential oil is proved to have the potential for its application in control of coccidiosis.