Reverse vaccinology assisted design of a novel multi-epitope vaccine to target Wuchereria bancrofti cystatin: An immunoinformatics approach

•in-silico techniques have been utilized to design a multi-epitope-based peptide vaccine to target W. bancrofti cystatin.•Molecular docking and dynamic simulation study postulated the role of vaccine in inducing protective innate immune responses.•Immune-simulation study figured out induced adaptive responses mediated by IgG, IgM and Th1 mediated responses.•Multi epitope-vaccine was also predicted for its RNA structure and cloned in pET30ax for further experimental validation.