Biocompatibility of Poly(3-Hydroxybutyrate)/Nano-Magnetite Composite Material for Bone Tissue Engineering Applications

The development of orthodontics and orthopedics requires new materials for guided bone regeneration. These materials must meet certain requirements for affinity with surrounding tissues and the ability to induce osteogenesis. Poly-3(hydroxybutyrate) (PHB) is a biocompatible and biodegradable material, actively used in different biomedical applications. Recent studies have shown piezoelectric properties of PHB. Since electrical signals have a huge impact on differentiation and cell growth, novel materials with piezoelectric properties could be effective tool for tissue regeneration. To enhance this effect in PHB polymer was combined with magnetic nanoparticles (MNs). The main component of MNs was magnetite (Fe3O4) synthesizing under different conditions using the magnetic separation method. Assessment of toxicity was obtained with composite films on mesenchymal stem cells (MSCs). Our work demonstrates that composites PHB/MNs have a potential to be used as a smart new material for bone tissue engineering applications.