Targeted Mutagenesis in Mice Using a Base Editor.
Methods in molecular biology (Clifton, N.J.)(2023)
摘要
Base editors, such as cytosine and adenine base editors, are composed of nickase Cas9 (nCas9) and deaminase and serve as Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-based enzymatic tools for specific nucleotide substitutions. They are mainly the most effective genome editing tools for introducing point mutations, such as C-to-T and A-to-G conversions. The enhanced base editor, a C-to-G base editor (CGBE), can perform other nucleotide substitutions, such as C-to-G conversions. Here, we introduce a method for generating mouse models with point mutations using a base editing system.
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关键词
Adenine base editor,CRISPR system,Cytosine base editor,Mouse model generation,Nucleotide substitution,Targeted mutagenesis
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