ODNA: Identification of Organellar DNA by Machine Learning

Motivation Identifying organellar DNA, such as mitochondrial or plastid sequences, inside a whole genome assembly, remains challenging and requires biological background knowledge. To address this, we developed ODNA based on genome annotation and machine learning to fulfill. Results ODNA is a software that performs organellar DNA sequence classification of a genome assembly by machine learning based on a pre-defined genome annotation workflow. We trained our model with 829,769 DNA sequences from 405 genome assemblies and achieved very high predictive performance (e.g., MCC of 0.61) on independent validation data, thus outperforming existing approaches significantly. Availability Our software ODNA is freely accessible as a web service at and can also be run in a docker container. The source code can be found at and the processed data at Zenodo (DOI: 10.5281/zenodo.7506483). ### Competing Interest Statement The authors have declared no competing interest.