Protocol for standardized intrathymic injection in mice
STAR PROTOCOLS(2023)
摘要
Currently available intrathymic injection techniques cause postoperative complications or difficulties in equipment acquisition. Here, we describe a standardized intrathymic injection protocol that requires only basic equipment with a minimally invasive procedure. We detail steps to identify injection sites for intrathymic delivery. We then describe how to visualize a successful intrathymic injection by including Indian ink in the injected solution.For complete details on the use and execution of this protocol, please refer to Tsai et al. (2022).(1)
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关键词
Developmental Biology,Immunology
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